wAtCh OUT

On Monday, Feb. 16, we had a LOT to do. We counted our irradiated soil plates and calculated the cfu/g, the percent survival and 5kGy, completed streaking our starch plates, and picked 10 more colonies, 11-20, and took pictures of them. We also checked for growth in our liquid cultures, scored them, and streaked them out using 10 microliters. Before we streaked the plates we had to vortex the tubes to insure that the everything was thoroughly mixed. When we counted our irradiated plates our (-1) plates were the most countable. Our (-2 and -3) plates had very little to no growth. They also had some contamination. I find it very interesting that we had very little growth on our (-2 and -3) plates at 5kGy. That makes me think that at the higher dosages we won't have any survival on those plates. It should also be noted that most of our growth on our (-1) plates occurred on 1/10 PCA and 1/100 PCA. To me, this seems like the organisms don't require a lot of nutrients to grow. The percent survival for the 1/10 PCA and the 1/100PCA plates were only 8.4% and 16.4%, respectively. I find it very interesting that Golbi had such high percentages of growth on all of the media. In addition, when we scored our liquid cultures we noticed that every tube had growth. So, we scored them from 1-4+. We took out 4 totally different tubes, one that would be representative of 1-4+. By having something to compare the other tubes to made it a lot easier. Most of our tubes had 2+ growth. Next, when we chose our 10 colonies to streak out, we chose 6 black and 4 pink. I don't know for the other groups, but for us, we had tons of black colonies on our plates. In looking at our irradiated soil plates you will see that most of our growth did in fact occur on our 1/10 PCA and 1/100 PCA plates. The other media had very little to no growth.

LRH - growth on 1/10 PCA after irradiation: LRH - growth on 1/100 PCA after irradiation:
LRH - growth on PCA after irradiation:
LRH - growth on MA after irradiation:

On Wednesday, Feb. 18, we had a LOT to do AGAIN. HaHaHaaa!! I arrived a few minutes late (16 to be exact) because I couldn't find my key. The entire time it had been in my purse!! But, on a heavier note, when I arrived I found my partners looking at our temperature and NaCl plates. We took note of the growth, 1-4+, and the color of the colonies on the plates. In looking at our plates we noticed that we had lots of growth across the board. Every temperature had lots of growth. It was very surprising that we had growth the on the plates stored in the 10 degrees centigrade incubator. The organisms that survived on these plates obviously come from or have some resistance to cold environments. In addition, our NaCl plates had lots of growth too. However, as the NaCl was increased (1,3,6,9) we noticed that growth decreased. There were some that did grow very well. I think that these organisms are from the Modestabacter genera. I'm not sure so someone if you know please correct me on this. We don't have results for our 1/10 PCA plates because the incubator went out for a while and went to 25 degrees centigrade. Maybe, JUST MAYBE, someone should buy a more expensive incubator and not be so CHEAP!! That was a JOKE!! HaHAahaha… Later that day, we made stock cultures from 23 , 25 degrees centigrade plates, and 3 (the 1/10 PCA) from the cold room.