Blog then beach...

On Monday, we plated our strains that were left in the dessicator for 42 days. We resuspended the cells in each well with 500 ul of liquid media. We left the liquid media in each well for 30 minutes and then repeatedly mixed the cells with the media for 30 secs by pipetting the solution up and down. We spotted the media each strain grew best on.
We Also ran a PCR on the DNA extracted from the three soil samples and the isolates from the 3 soil samples that showed up on the previous gel using the geo primers. Our group restreaked GB 20. The PCR instructions are:
98 C for 10 minutes to denature the DNA
90C for 5 minutes to cool and then add 0.5 ul of Taq polymerase
93C for 30 seconds to denature the DNA
51C for 30 seconds to anneal the primers
72 C for 2 minutes for elongation
-Repeat above steps 40 times
72 C for 10 minutes to extend elongation to make sure all fragments are the same size
4 C ??
The results of the gel showed that the three soil samples contain members of the Geodermatophilaceae family. The results were excellent with a single band at around 564. Also, the soil samples containing some of our strains showed a single band consistent with the Geodermatophilaceae family. Our GB-20 had two bands. I’m not quite sure why this would happen.
On Wednesday, we performed more carbon utilization tests using 16 different carbon sources. A control plate was also made for comparison. 10 ul of the strains were spotted on each of the carbon sources. Our previous carbon source plates were doing well.
We also purified the PCR products from our 26 strains.