On Monday we talked about......

the IJESEM paper and all that it entails. In the paper we will either have to write about a new species in one of the three genera we are working with or one species that we think represents a new genus. If we do have a new genus or strain WE can name it. I’m thinking something like Keithobacter, or something of that nature! A good example to look at when writing your paper would be the Ahrens and Moll, 1970 paper. It has the correct structure, although, the introduction needs to be longer. In the paper the required sections are: isolation and cultivation, colony and cell morophology, chemotaxonomy, phylogenetic analysis, physiology, and abstract. After, we plated out our dessication experiment, re-streaked our stock plates, and spotted the organisms used in the dessication experiment. Dr. Rainey made the PCR pre-mix which include x10 Buffer (80uL), Geosp1 (8uL), Geosp2 (8uL), dNTPs (80uL), and H2O (632 uL). We put 49.5uL into each tube along with 0.5uL of DNA. While in the PCR machine the first hot start was used to denature the DNA and a higher temperature. We put the taq in after because it also denatures at high temperatures.
On Wednesday nothing, and I mean NOTHING exciting happen. We were supposed to to re-streak our carbon source plates, plate more carbon sources, and run our DNA out on a gel. However, all of our time consisted of re-streaking our carbon. Then after that we had to streak a new batch of carbon sources. This took the entire 3.5 hours. My thumb started to go numb and I started to become nauseous after the first hour. Like I said Wednesday was unexciting and nothing happened.