here you go frederico!

On Monday, we conducted carbon utilization test on our 78 strains. Eugene prepared basal media prior to our lab meeting for us. Thank you Eugene! The basal media consist:
1) 11 g Yeast Extract (2.5g Yeast Extract/ Liter)
2) 1g Calcium Carbonate/Liter
a. The calcium carbonate is a buffer that neutralizes the acid produced by the organisms
3) 1% NaCl (10g NaCl/ Liter)
We added one of 16 carbon sources to this media. In addition, one set of plates was deemed the control and had no carbon source added to the basal media. We will use this plate as a comparison to see if there was more growth on the control plates or on the media containing the carbon sources. The carbon sources that we used are:
1. Control C
2. Arabinos AR
3. Dulictol DL
4. Galactose GA
5. Glucose GU
6. Glycerol GY
7. Inositol In
8. Lactose LA
9. Mannitol MN
10. Melezitose MZ
11. Melibiose MB
12. Raffinose RF
13. Rhannose RH
14. Ribose RI
15. Sucrose SU
16. Xylose XY
17. Xylan XN
By the way, I hope that all of you are aware of the fact that I made a mistake when typing up the carbon sources for everybody. The C is CONTROL not carbon.
We sported 10 ul of each strain onto each carbon sources. There are 6 strains/plate. Cells were washed with saline prior to spotting to remove any remnants of the previous media. The plates were then incubated at 25 C.
Also on Monday, we used our stock plates to streak strains that grew at 10C and incubated them at 4 C. All of our group’s 26 strains grew at 10 C and therefore were restreaked and incubated at 4 C except for strains 54, 58, 68, and 78.
On Wednesday, we observed the results of our pH plates. The strains were streaked on media at pH 4-10. A special thanks to everyone we came in over Mardi Gras to prepare these plates for us. We had some pretty interesting results and they are located on airset. Most notably, color changes were observed in several strains at the various pH’s. I am really excited about strain 69. At pH 6 it was a smooth black colony. However as the pH increased (7-10) it began to form a cream/peach color around its parameter. This made me think of Modestobacter versicolor. However, in the paper pH did not induce a color change. Nutrient conditions elicited the color change. Who knows! I’m still excited. Our plates containing strains 67,69,70, 75, and 76 are awesome. The color of the colonies of these plates look like it could be all three genera of interest. There is a orange colony (maybe Blastococcus), two black colonies (Geodermatophilus), a cream colony (Modestobacter multisepatus/versicolor), and a colony that is centrally dark with a cream parameter (Modestobacter versicolor),

pH 7 plate containing 67,69, 70, 75, and 76

Lastly on Wednesday, we extracted DNA from our three soil samples using the protocol on airset. 10g of each soil was used.
Remind me to tell yall on Monday a really great story that happened at the Circle K last night.