On Monday of this week, we first recorded our results from the 0, 3, 6, and 16 kGy irradiated samples of our 26 strands. We recorded our results into an excel sheet and found that most of our strand have a good tolerance for irradiation. Then we recorded our result from the starch hydrolysis experiment. We also recorded these results into an excel sheet. The results varied with some showing a strong presence for amylase activity, others weak, and others not at all. These result s will contribute greatly in determining what species of bacteria we have.
In the image of strand 78, the clear orange areas show the presence of amylases:
In the image of strand 78, the clear orange areas show the presence of amylases:
In the images of 3, 6, and 16 kGy for our cultures 54, 57, and 58 which grow on MA, show that all three stands can tolerate 3 kGy. But only 54 and 57 can survive 6 kGy, and none were able to live though the dosage of 16 kGy.
Growth of strains after exposure to 3kGy:
Growth of strains after exposure to 6kGy:
Growth of strains after exposure to 16kGy:
On Wednesday, we took our dreaded MID-TERM, which took the complete hour and a half. I found it fair and hope Dr. Rainey is lenient when grading them.
After the test, we streaked our 26 strands on pH plates ranging from 4 to 10. We will check these results in a couple of weeks
Note: the pH test was done by "spotting" 10ul of cell suspensions on media at different pH values not by "streaking"...
ReplyDeletethe 26 different cultures you are working with are called strains, rather than strands.
ReplyDeleteIn looking at your plates I noticed that at 3 kGy there are 2-3 different colonies and at 6kGy there is only one colony in each quadrant. I wonder why this is..
ReplyDelete